Services
Microscopes & Equipment
AFM | Confocal | Epifluorescence | Image Analysis | Light Sheet | Microdissection | Micropatterning | SMLM | SIM | Super Resolution | Two-Photon | 3D Printing
Super Resolution
Olympus IXplore SpinSR10 + Microdissection
The Olympus IXplore SpinSR10 spinning disk confocal super resolution microscope is designed to provide super resolution (down to 120nm XY resolution) and prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching.
The microscope has been adapted with a Rapp OptoElectronic UGA-42 Caliburn 355/42 pulsed laser (355nm, 1kHz, 42µJ/pulse) to perform ablation and microdissection.
Objectives
• 4x/0.16 UPLXAPO | Air | WD: 13mm
• 10x/0.40 UPLXAPO | Air | WD: 3.1mm
• 20x/0.80 UPLXAPO | Air | WD: 0.6mm
• 40x/1.30 UPLFLN | • Oil | WD: 0.2mm
• 60x/1.42 UPLXAPO | • Oil | WD: 0.15mm
• 100x/1.30 UPLFLN | • Oil | WD: 0.2mm
• 100x/1.50 UPLAPO | • Oil | WD: 0.12mm
Camera
· Resolution 2304px x 2304px
· Pixel Size: 6.5µm x 6.5µm
· Effective Area: 14.976mm × 14.976mm
· Bit depth: 16bit
Laser Lines
405 nm | Diode | 50mW
488 nm | Diode | 100mW
561 nm | Diode | 100mW
640 nm | Diode | 100mW
Confocal Filters
Ex: Multi-band 405 - 488 - 561 - 640
Em: Band-pass 447/60 | 525/50 | 586/20 | 617/73 | 655/40 | 685/40
Widefield Light Source
Widefield Filters
Ex: BP 352-402 | BS: 4090 | Em: LP 409
Ex: BP 460-480 | BS: 495 | Em: BP 495-540
Ex: BP 530-550 | BS: 570 | Em: LP 575
Ex: BP 565-585 | BS: 595 | Em: BP 600-690
Manuals đź”—
STED Microscope for SUSHI
A custom-built Stimulated Emission Depletion (STED) microscope designed for super-resolution shadow imaging (SUSHI) experiments. The microscope is mounted on an optical bench and based on a Leica DMI-8 body.
Single Molecule Localization Microscopy (SMLM)
ONI Nanoimager
The ONI Nanoimager is a compact microscope suitable for Single-molecule localization microscopy (SMLM) experiments, including dStorm, PALM or DNA-PAINT, Single Particle Tracking (SPT), smFRET, HILO or TIRF.
This easy-to-use microscope permits a quick start for imaging, no alignment or calibrations required and real time data build up making it a flexible device for different imaging & illumination modes for either fixed and live cell imaging, and programmable workflows.
Objectives
• 100x/1.45 UPLXAPO | • Oil | WD: 0.13mm
Camera
· Resolution 2048px x 2048px
· Pixel Size: 6.5µm x 6.5µm
· Effective Area: 13.312mm x 13.312mm
· Bit depth: 16bit
Laser Lines
405 nm | Diode | 18mW
488 nm | Diode | 1W
561 nm | Diode | 750mW
638 nm | Diode | 1W
Manuals đź”—
Confocal & Two-Photon
Leica Confocal TCS SP5 + Two-Photon
The Leica TCS SP5 is an advanced confocal microscope suitable for scanning fixed samples and live cell imaging.
The microscope is equipped with 3 PMT, 2 Hybrid and 2 APD detectors and has been adapted with a Mai-Tai DeepSee Infrared Laser to perform Multiphoton Microscopy and with a Becker & Hickl Simple-Tau 830 TCSPC system to carry out FLIM, FCS and FCCS experiments.
Objectives
• 10x/0.22 HI PL I Ph1 | Air | WD: 7.8mm
• 10x/0.30 HC PL FLUOTAR | Air | WD: 11mm
• 20x/0.30 HI PL I Ph1 | Air | WD: 3mm
• 20x/0.70 HC PL APO CS | Air | WD: 0.59mm
• 40x/0.60 HCX PL FL L CORR | Air | WD: 3.3-1.9mm
• 40x/0.75 HCX PL APO U-V-I | Air | WD: 0.28mm
• 40x/0.85 HCX PL APO CS CORR | Air | WD: 0.24mm
• 63x/1.20 W HCX PL APO CS CORR | • Water | WD: 0.22mm
• 63x/1.30 Glyc HCX PL APO CS | • Glyc | WD: 0.28mm
• 100x/1.30 Oil HCX PL FLUOTAR | • Oil | WD: 0.13mm
Detectors
3 PMT
2 GaAsP Hybrid Detectors
2 APD
Laser Lines
405 nm | Pulsed Diode | 3mW (avg.)
458 nm | Argon | 65mW
476 nm | Argon | 65mW
488 nm | Argon | 65mW
496 nm | Argon | 65mW
514 nm | Argon | 65mW
543 nm | HeNe | 5mW
633 nm | HeNe | 10mW
690-1040nm | Ti:Sapphire | 2.4W (avg.)
Filters
Acousto Optical Beam Splitter (AOBS)
Widefield Filters
Ex: BP 340-380 | BS: 400 | Em: LP 425
Ex: BP 450-490 | BS: 510 | Em: LP 515
Ex: BP 515-560 | BS: 580 | Em: LP 590
Ex: BP 590-650 | BS: 660 | Em: BP 663-738
Manuals đź”—
Structured Illumination Microscopy (SIM)
Zeiss Axio Observer 7 + Apotome 3
The Zeiss Axio Observer 7 is a standard widefield fluorescence microscope equipped with a Zeiss Apotome 3, capable of producing optical sections of a fluorescent specimen by removing the out-of-focus light using structured Illumination.
Objectives
• 10x/0.25 LD A-Plan Ph1 | Air | WD: 8.5mm
• 20x/0.40 LD Plan-Neofluar Corr Ph2 | Air | WD: 7.4-8.4mm
• 20x/0.80 PL APO | Air | WD: 0.55mm
• 40x/0.55 LD A-Plan Ph2 | Air | WD: 2mm
• 40x/0.95 Plan Apo Corr | Air | WD: 0.25mm
• 63x/1.40 Oil Plan Apo | • Oil | WD: 0.19mm
Camera
· Resolution 1936px x 1460px
· Pixel Size: 4.54µm x 4.54µm
· Effective Area: 8.80mm x 6.60mm
· Bit depth: 14bit
Light Source
385 nm | 475 nm | 555 nm | 630 nm
Filters
Ex: BP 370-410 | BS: 420 | Em: BP 430-470
Ex: BP 450-490 | BS: 495 | Em: BP 500-550
Ex: BP 538-562 | BS: 570 | Em: BP 570-640
Ex: BP 625-655 | BS: 660 | Em: BP 665-715
Manuals đź”—
Epifluorescence
Nikon Eclipse TE-2000
The Nikon Eclipse TE-2000 is a standard epifluorescence microscope, capable of acquiring fixed specimen and live cell imaging.
Objectives
• 10x/0.25 CFI Plan Fluor | Air | WD: 16mm
• 20x/0.40 LWD | Air | WD: 3mm
• 20x/0.45 ELWD S Plan Fluor | Air | WD: 8.2-6.9mm
• 40x/1.30 Oil S Fluor | • Oil | WD: 0.22mm
• 60x/1.49 Oil Plan APO TIRF | • Oil | WD: 0.12mm
Camera
· Resolution 1392px x 1040px
· Pixel Size: 6.45µm x 6.45µm
· Effective Area: 9.00mm x 6.70mm
· Bit depth: 12bit
Light Source
Filters
Ex: BP 436/10 | BS: 455 | Em: LP 475
Ex: BP 482/35 | BS: 505 | Em: LP 520
Ex: BP 531/40 | BS: 562 | Em: BP 590/40
Ex: BP 562/40 | BS: 593 | Em: BP 624/40
Manuals đź”—
Atomic Force Microscopy (AFM)
JPK Nanowizard UltraSpeed AFM
Mounted on a Zeiss Axio Observer 7 equipped with a Zeiss Apotome 3, the JPK Nanowizard UltraSpeed Atomic Force Microscope (AFM) enables the tracking of changes in the specimen in real time.
Objectives
• 10x/0.25 LD A-Plan Ph1 | Air | WD: 8.5mm
• 20x/0.40 LD Plan-Neofluar Corr Ph2 | Air | WD: 7.4-8.4mm
• 20x/0.80 PL APO | Air | WD: 0.55mm
• 40x/0.55 LD A-Plan Ph2 | Air | WD: 2mm
• 40x/0.95 Plan Apo Corr | Air | WD: 0.25mm
• 63x/1.40 Oil Plan Apo | • Oil | WD: 0.19mm
Cameras
· Resolution 2464px × 2056px
· Pixel Size: 3.45µm x 3.45µm
· Effective Area: 8.50mm x 7.10mm
· Bit depth: 14bit
· Resolution 1920px × 1200px
· Pixel Size: 5.86µm x 5.86µm
· Effective Area: 11.25mm x 6.33mm
· Bit depth: 12bit
Light Source
385 nm | 475 nm | 555 nm | 630 nm
Filters
Ex: BP 370-410 | BS: 420 | Em: BP 430-470
Ex: BP 457-484 | BS: 495 | Em: BP 500-550
Ex: BP 450-490 | BS: 495 | Em: BP 570-640
Ex: BP 538-562 | BS: 570 | Em: BP 572-642
Manuals đź”—
Micropatterning
Alvéole Primo 2 Micropatterning System
Mounted on a Nikon Ti2 widefield fluorescence microscope, the Alvéole Primo 2 allows, all in a single device, micropatterning, hydrogel polymerization and microfabrication, to create bespoke in vitro cellular microenvironments or to get better cell models for cell biology experiments or electron cryotomography studies.
Objectives
• 10x/0.25 CFI Plan Fluor | Air | WD: 16mm
• 20x/0.40 LWD | Air | WD: 3mm
• 20x/0.45 ELWD S Plan Fluor | Air | WD: 8.2-6.9mm
• 40x/1.30 Oil S Fluor | • Oil | WD: 0.22mm
• 60x/1.49 Oil Plan APO TIRF | • Oil | WD: 0.12mm
Camera
· Resolution 2048px x 2048px
· Pixel Size: 6.5µm x 6.5µm
· Effective Area: 13.312mm x 13.312mm
· Bit depth: 16bit
Light Source
Filters
Ex: BP 375/28 | BS: 415 | Em: BP 460/60
Ex: BP 470/40 | BS: 500 | Em: BP 535/50
Ex: BP 540/25 | BS: 565 | Em: BP 605/55
Manuals đź”—
Light Sheet
Light Sheet Microscope
... Installation in Progress ...
A custom-built Selective Plane Illumination Microscope (SPIM), for living or clarified organisms, for imaging at fixed magnification with water dipping objectives.
Image Analysis
Image Analysis Workstation Pro
CPU: Intel Xeon W-2255 3.70GHz (10 Cores)
RAM: 512GB
Software: Bitplane Imaris 9.7 Core Facility, Huygens Essentials 21.04, Matlab R2021a, JPK Data Processing, FiJi/ImageJ, QuPath, Icy, Ilastik, CellProfiler, Leica LAS X Core, Nikon NIS-Elements viewer, Zeiss Zen Lite, FLIMFit, SPCImage, Agave 3D, Blender...
Image Analysis Workstation Lite
CPU: Intel Xeon E5-1650 3.2Ghz (6 Cores)
RAM: 32GB
GPU: Nvidia GTX970 4GB
Software: Matlab R2017b, JPK Data Processing, FiJi/ImageJ, QuPath, Icy, Ilastik, CellProfiler, Leica LAS X Core, Nikon NIS-Elements viewer, Zeiss Zen Lite, FLIMFit, SPCImage, Agave 3D, Blender...
3D Printing
Formlabs Form 3+
Form 3+ is a resin 3D printer that uses improved SLA technology.
The Form 3+ is characterized by creating prototypes on a larger scale with a printing volume of 145x145x185 mm and by manufacturing final pieces with industrial quality.
The LFS (Low Force Stereolithography) printing technology uses linear lighting and a flexible tank to convert liquid resin into solid parts. The part in charge of lighting is the LPU (Light Processing Unit), a single module that contains all the parts (lenses, mirrors and laser) that are needed to solidify the resin during the manufacturing of a part with a laser power of 250 mW and a precision in an XY plane of 25 ÎĽm.
This system offers sharp and clean characteristics as the high-power laser passes through a filter and ensures a concentrated laser spot. The parabolic mirror ensures that the projection of the laser on the manufacturing plane is always perpendicular, which ensures maximum printing quality and the exact reproduction of the design to be manufactured.
MicroscopĂa Ă“ptica Avanzada | MicroscopĂa Confocal | MicroscopĂa de Super ResoluciĂłn | MicroscopĂa de 2 Fotones | MicroscopĂa de Fuerza AtĂłmica | Micropatronaje | ImpresiĂłn 3D | Análisis de Imagen en MicroscopĂa