The Basque Resource for Advanced Light Microscopy (BRALM) of the Instituto Biofisika (IBF), a joint research center of the University of the Basque Country (EHU) and the Spanish Research Council (CSIC), is an open access facility offering state-of-the-art advanced light microscopy equipment and image analysis resources. Supported by the Fundación Biofísica Bizkaia (FBB) and by the Department of Education of the Basque Government, BRALM focuses on quantitative imaging of cellular events through the implementation of sophisticated imaging modes and data analysis. BRALM take part in the Bilbao Advanced Light Microscopy Node of the EuroBioImaging ERIC network.